Journal: Molecules

Article Title: Naringin Exhibited Therapeutic Effects against DSS-Induced Mice Ulcerative Colitis in Intestinal Barrier–Dependent Manner

doi: 10.3390/molecules26216604

Figure Lengend Snippet: Effect of naringin on the expression of inflammatory cytokines in ulcerative colitis mice. ( A ) TNF-α, IL-6 and IL-1β distribution in the colons of each group (immunofluorescence analysis, 200×). ( B ) Concentration of TNF-α in the blood serum. ( C ) Concentration of IL-6 in the blood serum. Data are presented as means ± SD ( n = 3). ## p < 0.01, compared with the control group; * p < 0.05 and ** p < 0.01, compared with the model group.

Article Snippet: Rabbit polyclonal antibodies for TNF-α, IL-6, IL-1β, COX-2, iNOS, Occludin, ZO-1, goat anti-rabbit IgG secondary antibodies and Texas Red conjugated goat anti-rabbit antibody were obtained from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Concentration Assay, Control

Journal: Neural Regeneration Research

Article Title: Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury

doi: 10.4103/1673-5374.303035

Figure Lengend Snippet: Expression levels of proteins associated with the blood-brain barrier and the regulation of inflammation-related protein in the penumbra area of rats with DM complicated with TBI. (A–E) Immunopositivity (arrows) of HO-1, MPO, occludin, and ZO-1 proteins (A) and the percentage of positive cells (B–E). The results showed that compared with those in the other groups, the expression levels of HO-1 and MPO in the TBI + DM + L-NIO group increased significantly, whereas the expression levels of ZO-1 and occludin decreased significantly. Scale bars: 400 µm and 40 µm (as labeled). Data are expressed as the mean ± SD ( n = 6). * P < 0.05 (one-way analysis of variance followed by Bonferroni post hoc test). DM: Diabetes mellitus; HO-1: heme oxygenase-1; L-NIO: N(5)-(1-iminoethyl)-L-ornithine; MPO: myeloperoxidase; TBI: traumatic brain injury; ZO-1: zonula occludens-1.

Article Snippet: The sections were blocked in phosphate-buffered saline containing 1.5% normal goat serum and 1% bovine serum albumin for 2 hours at room temperature, incubated with rabbit anti-heme oxygenase-1 (HO-1, 1:100; Cat# AF5393; Affinity Biosciences, Cincinnati, OH, USA) (Bharucha et al., 2010), rabbit anti-myeloperoxidase (MPO, 1:200; Cat# PAA601Ra01; Cloud-Clone Corp., Houston, TX, USA) (Jang and Rabb, 2009), rabbit anti-occludin (1:200; Cat# DF7504; Affinity Biosciences) (Yu et al., 2005), and rabbit anti-zonula occludens-1 (ZO-1, 1:200; Cat# PAC262Ra01; Cloud-Clone Corp.) (Jiao et al., 2011) antibodies at 4°C overnight.

Techniques: Expressing, Labeling